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Isolation of brucella spp from milk of brucellosis positive cows in São Paulo and Minas Gerais states Braz. J. Vet. Res. Anim. Sci.
LANGONI,Helio; ICHIHARA,Sílvio Massaru; SILVA,Aristeu Vieira da; PARDO,Renata Bonini; TONIN,Flávia Bechelli; MENDONÇA,Lia Jeanne Pereira; MACHADO,José Aparecido Dorta.
Brucellosis is a chronic zoonosis that plays an important role in Public Health. Considering the lack of data in Brazil regarding its presence in raw milk and non-pasteurized dairy products, we determined the presence of brucellae in milk from brucellosis positive animals. The slide agglutination test (SAT), tube agglutination test (TAT) and TAT treated with 2-mercaptoethanol were used to identify positive animals in studied herds. For 3 days, 300 ml milk samples/cow (75 ml/teat) were collected from all productive quarters of the positive animals. These were mixed and centrifuged. Part of the pellet and of the supernatant were inoculated in Farrel and Brodie-Sinton (BS) media supplemented with antimicrobial agents. The inoculated plates and tubes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Brucella abortus; Cows; Brucellosis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600004
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Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil Acta Amazonica
PAZ,Giselle Souza da; ROCHA,Katarine de Souza; LIMA,Michele de Souza; JORGE,Ediene Moura; PANTOJA,José Carlos Figueiredo; MORAES,Carla Cristina Guimarães de; LANGONI,Helio.
Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira spp.; Brucella spp.; Domestic canids; Serology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672015000300265
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